PREGNANCY-ASSOCIATED PLASMA PROTEIN-A2 (PAPP-A2) POLYNUCLEOTIDES

摘要

The present invention provides pregnancy associated plasma protein A2 (PAPP-A2), its nucleotide and amino acid sequences, antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-encoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, and methods for screening for pathologies in pregnant and non-pregnant patients. Methods for screening for altered focal proliferation states in pregnant and/or non-pregnant patients, which include detecting levels of PAPP-A2, are also described.

主权项

1. An isolated polypeptide which comprises an amino acid sequence at least 95% identical to residues 234-1791 of SEQ ID NO:2 but differs therefrom solely by
(a) deletion of 1-10 residues from, or addition of 1-10 residues to, the amino terminal; and/or (b) deletion of 1-10 residues from, or addition of 1-10 residues to, the carboxy terminal; and/or (c) one or more conservative substitutions;wherein said polypeptide has a proteolytic activity against Insulin Like Growth Factor Binding Protein 5 (IGFBP-5).