Systems and Methods for Sequencing Peptides by Mass Spectrometry

摘要

The number of atoms present in an ion of a molecule is identified using a mixture of different forms of the molecule. A mass spectrometer analyzes a mixture of at least two forms of the molecule using one or more ion scans producing a mass spectrum. The first form of the molecule includes a first combination of isotopes of one or more elements. The second form of the molecule includes a second combination of isotopes of the one or more elements. A first peak and a second peak that differ in mass by a multiple of a mass difference between the first combination of isotopes and the second combination of isotopes are located in the mass spectrum. The number of atoms of the one or more elements present in an ion of the molecule is identified from a mass difference between the first peak and the second peak.

主权项

1. A system for identifying the number of atoms present in an ion of a molecule using a mixture of different forms of the molecule, comprising:
a mass spectrometer that analyzes a mixture of at least two forms of a molecule using one or more ion scans across a mass range and produces a mass spectrum from the one or more ion scans for the mass range, wherein a first form of the molecule includes a first combination of isotopes of one or more elements, and wherein a second form of the molecule includes a second combination of isotopes of the one or more elements; and a processor in communication with the mass spectrometer that locates a first peak and a second peak in the mass spectrum that differ in mass by a multiple of a mass difference between the first combination of isotopes and the second combination of isotopes, and identifies a number of atoms of the one or more elements present in an ion of the molecule from a mass difference between the first peak and the second peak.