| 发明名称 |
System and Method for Emulsion Breaking |
| 摘要 |
A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution. |
| 申请公布号 |
US2015133649(A1) |
申请公布日期 |
2015.05.14 |
| 申请号 |
US201414536429 |
申请日期 |
2014.11.07 |
| 申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
REED Brian;Ball James A. |
| 分类号 |
B01D17/04;C07H21/00;C07H1/06 |
主分类号 |
B01D17/04 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of recovering a bead support from an emulsion, the method comprising:
supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5 and at least 0.01; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution. |
| 地址 |
Carlsbad CA US |
