CELL-BASED GENOMIC RECORDED ACCUMULATIVE MEMORY

摘要

The present invention relates to a cell based genomic Recorded Accumulative Memory (geRAM) system (also referred to herein as Genomically Encoded Memory (GEM)) for recoding data (i.e., changes in nucleic acid sequences in cellular DNA in response to physical and/or chemical signal(s)) from the cellular environment.

主权项

1. A recombinant cell-based data sensing and recording system comprising a cell comprising at least one nucleic acid sequence encoding at least one directed endonuclease specific to at least one target sequence located within the cell wherein the at least one directed endonuclease is operably linked to at least one inducible promoter, wherein the at least one inducible promoter is responsive to at least one signal of interest originating from outside or inside of the cell, and wherein upon inducement of the promoter in response to the signal, the promoter causes expression of the directed endonuclease wherein the expressed directed endonuclease binds to, and cuts the target sequence located within the cell, wherein the cell is capable of repairing the cut in the target sequence and wherein the repair of the target sequence introduces at least one error in the target sequence, wherein the presence of the at least one error in the target sequence provides a data record that the cell was exposed to the at least one signal of interest originating from outside or inside the cell.