Purification of Biological Molecules

摘要

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.

主权项

1. A process for the purification of a target molecule comprising the steps of:
a) providing a sample comprising the target molecule and one or more impurities; b) adding at least one precipitant to the sample and removing one or mom impurities, thereby to recover a clarified sample; c) subjecting the clarified sample from step (b) to a bind and elute chromatography step comprising at least two separation units, thereby to obtain an eluate comprising the target molecule; and d) subjecting the eluate to flow-through purification comprising use of two or more media; wherein at least two steps are performed concurrently for at least a portion of their duration and wherein the process comprises only one bind and elute chromatography step.