METHOD FOR INTEGRIN LIGAND DISCOVERY

摘要

This invention relates generally to integrin ligand discovery and to a method of integrin ligand discovery based upon induction of ligand-induced epitopes. Such ligands have the potential to be active agent as anti-inflammatory, anti-angiogenesis and/or anti-thrombotic agents and for the treatment of integrin mediated diseases and/or conditions.

主权项

1. A method for determining whether a compound of unknown integrin activity is a competitive ligand of an integrin molecule comprising exposing said integrin molecule to a compound of unknown integrin activity detection of the integrin ligand binding affinity of a compound of unknown integrin activity, allowing a sufficient time for said compound to bind to said integrin and expose at least one ligand induced epitope if said compound is a ligand of said integrin; exposing said integrin molecule to an antibody comprising a reporter which is specific for said ligand induced epitope and determining whether said compound is a ligand of said integrin by measuring the concentration of antibody bound to said integrin and comparing it to at least one control measurement.