| 主权项 |
1: A method for identifying the varieties of hops, the method comprising the steps of:
(i) amplifying fragments of DNAs derived from hops to be tested, the fragments which each comprise a variety identification region, wherein the variety identification region comprises an identification marker composed of at least one single nucleotide polymorphism (SNP) that differs among hop varieties; (ii) identifying the genotype of the single nucleotide polymorphism in each of the DNA fragments amplified at step (i); (iii) comparing the genotype of the single nucleotide polymorphism in the variety identification region in each of the DNAs from the hops to be tested, as identified at step (ii), with the genotype of a single nucleotide polymorphism in a corresponding region in a DNA from a known hop variety, to thereby analyze whether the hop varieties to be tested and the known hop variety are concordant or discordant for the genotype of the single nucleotide polymorphism in the region in question; and (iv) identifying the varieties of the hops to be tested, on the basis of the analysis results obtained at step (iii). |
