METHODS FOR ISOLATING A PEPTIDE METHODS FOR IDENTIFYING A PEPTIDE

摘要

The present invention is directed to methods, for example phage display assays, for bioengineering peptides that bind to individual distinct nucleotides. Also provided are peptides engineered by such methods. Specifically, cyclic peptides that bind individual distinct nucleotides are provided herein.

主权项

1. A method for isolating a peptide, comprising:
contacting a first peptide library comprising a first subset of peptides with a first set of nucleotides comprising a heterogeneous population of nucleotides other than a pre-selected nucleotide, to identify a first sub-library of peptides comprising a second subset of peptides that do not bind to the first set of nucleotides; contacting the first sub-library of peptides with a homogeneous population of the pre-selected nucleotide, to identify a second sub-library of peptides that bind to the pre-selected nucleotide; and isolating at least one peptide from the second sub-library of peptides; wherein the first peptide library comprises a sub-library preselected from an initial peptide library by at least two rounds of biopanning.