LOW ACIDIC SPECIES COMPOSITIONS AND METHODS FOR PRODUCING AND USING THE SAME

摘要

The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

主权项

1. A method for producing a composition comprising adalimumab, the method comprising:
culturing a cell producing adalimumab in a cell culture media comprising an amount of one or more amino acid sufficient to produce a composition comprising adalimumab wherein the composition comprises less than 10% total acidic species of adalimumab.