NEW METHODS TO PRODUCE ACTIVE TERT

摘要

The present invention relates to a method to reproducibly produce large amounts of soluble and active telomerase reverse transcriptase (TERT) by expressing this enzyme in yeast cells. High yield of recombinant active TERT has been obtained by controlling specific parameters of the purification process. The TERT protein thus obtained displays telomerase activity with in vitro transcribed TR, opening the way to high throughput chemical screening of telomerase assembly inhibitors and therapeutic applications.