CHIP, AND METHOD FOR MEASURING SURFACE PLASMON-ENHANCED FLUORESCENCE

摘要

　In the present invention, a chip is prepared having a liquid storing part for retaining a liquid, a metal film including a diffraction grating arranged in the bottom part of the liquid storing part, and a capturing body immobilized on the diffraction grating. A sample liquid is introduced to the liquid storing part, and the sample liquid in the liquid storing part is then substituted with another liquid. Excitation light is radiated to the diffraction grating from the direction of an opening in the liquid storing part so as to generate surface plasmon resonance in the diffraction grating, and fluorescence, emitted from a fluorescent substance for labeling the substance to be detected, is detected. The surface area of the liquid in the liquid storing part when fluorescence is detected is greater than the surface area of the sample liquid in the liquid storing part when the sample liquid is introduced.