运用比较基因组学快速鉴定荠菜抗白粉病基因

摘要

本发明是快速鉴定荠菜抗白粉病基因；涉及到植物比较基因组学，遗传学和生物信息学等学科知识，属于植物生物技术科学领域。该发明主要步骤为：1)荠菜全基因组序列的下载及MLO型基因的采集；2)MLO型基因的鉴定；3)MLO型基因系统发育关系；4)MLO型白粉病基因的比对。该发明有效的缩短了荠菜白粉病基因挖掘周期，有利于白粉病基因的快速鉴定；通过鉴定的候选白粉病基因开发相应的共分离功能性标记(SNP、SCAR等)，还可以快速的用于抗白粉病基因的分子标记辅助选择，准确性高；结合其它抗病基因分子标记可进行多抗性育种材料的创制，缩短育种年限，提高育种效率；为阐述荠菜抗白粉病分子机制奠定了基础。

主权项

荠菜抗白粉病基因，其特征在于选自下列4个基因或其之一：carubv10022893m.g氨基酸序列：MAIKERTLEETPTWAVAVVCFVLLFISIMIEYFLHFVGHWFKKKHKKALYEALEKVKAELMLLGFISLLLVVLQDPVSQICIPRKVASTWHPCTHHQEMVKYGKDYVDDGRKILEDFDSTDVFGPRRSLATKGYDKCAEKGKVALVSAYGIHQLHIFIFVLAVFHILYCIITYALGKTKMKKWKSWEKETKTIEYQYANDPERFRFARDTSFGRRHLNMWSKSSVTLWITCFFRQFFGSVTKVDYLTLRHGFIMAHLPAGSAARFDFQKYIQRSLEEDFTVVVGISPLIWCIAVLFILTNTNGWASYLWLPFIPLLVILVVGAKLQMIISKLGLRIQDKGDVVKGAPVVEPGDDLFWFGRPRLILFLIHLVLFTNAFQLAFFVWSTYEFTLRNCFHHKNEDIAIRITMGVLIQVICSYITLPLYALVTQMGTSMRPTIFNDRVANALKKWHHTAKKQTKHGQSGSNTPHSSRPATPTHGMSPVHLLHNYHNRSSLDQQTSFTASPSPPRFSDYSGHGHGHHQFFDPESQNHSYQREITDSEFSNSHHPHTDVASPVREEMQIVEHVKVDLPEFTFKK核苷酸序列：ATGGCGATAAAAGAGCGAACACTGGAGGAGACACCAACATGGGCTGTTGCTGTTGTTTGCTTCGTTCTCCTTTTCATTTCCATCATGATCGAATATTTCTTGCACTTTGTTGGTCACTGGTTTAAAAAGAAGCACAAGAAAGCTTTATATGAAGCTCTTGAAAAGGTTAAAGCAGAATTGATGCTACTGGGATTCATATCGCTTCTATTGGTGGTATTGCAAGATCCAGTCTCTCAGATTTGCATTCCAAGAAAAGTTGCTTCGACTTGGCATCCTTGTACCCACCATCAAGAAATGGTTAAATACGGTAAAGATTATGTCGACGATGGCCGCAAGATTCTTGAAGACTTTGACTCCACCGACGTTTTTGGTCCTCGCCGCAGTTTAGCCACCAAAGGTTATGACAAATGCGCAGAAAAGGGAAAAGTAGCATTAGTATCTGCATATGGTATCCACCAGCTGCATATATTCATCTTTGTGCTTGCTGTTTTTCATATCCTCTACTGCATTATAACCTATGCTTTGGGGAAGACCAAGATGAAGAAATGGAAGTCATGGGAGAAAGAGACCAAAACAATTGAGTACCAATATGCCAATGATCCCGAGAGGTTCAGATTTGCAAGAGACACATCGTTCGGACGTAGACATTTGAACATGTGGAGCAAGTCTTCCGTTACCCTCTGGATTACATGTTTCTTCAGACAATTCTTTGGATCAGTGACAAAAGTGGATTATCTTACACTAAGACATGGCTTTATTATGGCGCATTTGCCAGCAGGAAGTGCAGCTCGTTTCGATTTCCAAAAATACATTCAAAGATCTTTGGAAGAAGATTTCACGGTGGTGGTCGGCATAAGTCCATTGATATGGTGCATTGCTGTCTTATTCATATTGACCAACACAAATGGATGGGCTTCATATCTTTGGTTGCCCTTCATCCCATTGCTTGTGATATTGGTAGTAGGAGCAAAACTTCAAATGATAATATCCAAGTTAGGATTAAGGATTCAAGACAAAGGAGATGTGGTTAAAGGAGCTCCTGTGGTTGAACCGGGTGATGATCTCTTCTGGTTTGGTCGTCCTCGTTTGATCCTCTTCCTCATCCACTTGGTCCTTTTCACGAATGCATTTCAACTGGCTTTCTTCGTTTGGAGCACTTACGAATTCACACTCCGAAACTGCTTCCACCACAAAAACGAAGATATTGCAATTAGGATCACCATGGGGGTATTAATACAAGTTATATGCAGCTACATCACTCTACCTCTCTATGCTCTTGTTACTCAGATGGGAACTTCAATGAGGCCGACCATATTCAACGATAGGGTAGCAAATGCATTGAAGAAATGGCACCACACAGCCAAGAAACAGACGAAACATGGACAGTCGGGATCTAACACACCTCACTCTAGCCGCCCTGCTACGCCAACTCATGGCATGTCACCGGTGCATCTCCTCCACAACTACCATAACCGCAGCAGCCTCGACCAACAAACCAGCTTCACGGCCTCTCCTTCTCCTCCTAGATTCTCTGATTATAGCGGCCATGGCCATGGCCATCATCAGTTCTTCGACCCTGAATCTCAGAATCACTCTTACCAGCGTGAGATCACAGATTCTGAATTCAGCAACAGTCATCATCCCCATACTGACGTTGCAAGTCCTGTTAGAGAAGAGATGCAGATTGTTGAGCATGTCAAGGTTGATTTGCCTGAATTTACCTTCAAGAAGTAGcarubv10009086m.g氨基酸序列：MADATLEETSTWTVAVVCFVLLLISIALEHLLHTLGTWFKKKHKSDLFEALEKVKAELMLLGFISLLLTIAQAPISHICISKKLASIMQPCSPAQAAKLKADRAKKEGGYGEKPGGKLLLELAEFYIPRRSLAGKVENTCEKKGKVPFVSYYGIHQLHIFIFVLAVVHIIYCIVIYALGKTKMRRWQPWEEEAKSIEYQFSNDPERFRFARDTSFGRRHLSFWSKRSVTLWTVCFFRQFFGSITKVDYLTLRHGFITAHFAPGQSSYDFRKLIQRTLEKDFKTVVEISPVIWFVAVLFLLTNTSGLYSHFWLPFIPLVVSLIVGTKLEVIITQLGQSIQEKDEIVRGAPVVRPKDDLFWFSKPRFVLFLIHLVLFTNAFQLAFFAWSTFSFGIKNCFHASIPDVVTRLVSFCTDTLQLCDSSTLCTCYSHGYSNEANSIQRRSSHSIREVASDGEEPD核苷酸序列：ATGGCAGACGCGACTTTAGAGGAGACCTCTACGTGGACAGTAGCTGTGGTTTGCTTCGTCTTACTACTCATTTCGATTGCGCTCGAACATTTGCTTCACACACTTGGAACCTGGTTTAAAAAGAAGCACAAAAGCGATCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGTTGTTGGGATTCATATCACTACTCCTAACAATTGCACAAGCACCAATCTCACATATTTGCATCTCCAAGAAACTTGCATCGATAATGCAACCTTGCAGTCCCGCCCAAGCAGCGAAATTAAAGGCCGATAGGGCAAAGAAAGAAGGTGGATATGGAGAAAAACCCGGTGGAAAACTTCTTCTTGAGCTGGCTGAATTTTATATCCCTAGAAGAAGTTTAGCCGGCAAAGTTGAGAACACATGCGAAAAGAAGGGGAAAGTGCCTTTTGTATCTTATTATGGAATCCACCAGCTGCATATATTCATCTTCGTGCTCGCAGTGGTTCATATTATTTACTGCATTGTTATTTATGCTCTCGGAAAGACCAAGATGAGAAGGTGGCAGCCGTGGGAGGAGGAGGCAAAGTCAATAGAGTATCAGTTTTCCAACGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCTTTTGGGAGAAGACATCTCAGTTTCTGGAGCAAGAGGAGTGTTACACTATGGACTGTGTGTTTTTTCAGACAGTTCTTTGGATCCATCACCAAAGTTGATTACTTAACATTAAGACATGGTTTTATCACGGCGCATTTCGCTCCCGGCCAATCAAGCTACGATTTCCGTAAGTTAATCCAGAGAACGTTAGAGAAAGACTTCAAAACCGTTGTTGAAATCAGTCCTGTTATCTGGTTTGTCGCCGTGCTATTCCTCTTGACCAATACAAGTGGGTTATATTCTCACTTCTGGTTACCATTCATTCCACTAGTCGTAAGTCTAATCGTTGGAACAAAGCTTGAAGTCATAATAACACAATTGGGTCAAAGTATCCAAGAGAAAGACGAAATAGTGAGAGGCGCCCCTGTGGTTCGGCCTAAAGATGACCTCTTCTGGTTTAGCAAGCCACGTTTCGTTCTTTTCCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTTGCCTTCTTTGCCTGGAGCACGTTTTCATTCGGGATCAAGAATTGTTTCCATGCAAGCATTCCAGATGTGGTCACTAGACTTGTTAGCTTTTGTACAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCACATGGGTACTCAAATGAAGCCAACAGTATTCAACGAAGGAGTAGCCACAGCATTAGAGAAGTGGCATCAGACGGCGAAGAACCAGACTAAcarubv10008691m.g氨基酸序列：MANQVKERTLEETSTWAVAVVCFVLLFISIVLEHSIHKIGTWFRKKHKKALFEALEKVKAELMLLGFISLLLTIGQTPISNICISEQVASTMHPCSAAEEARKYHKKDPAKKEHGDGDGEKSGRRLLLELAESYIPRRSLATKGYDKCAEKGKVAFVSAYGIHQLHIFIFVLAVVHVIYCIVTYALGKTKMRRWKSWEEETKTIEYQYSNDPERFRFARDTSFGRRHLNFWSKSSVTLWVVCFFRQFFGSVTKVDYLALRHGFIMAHLAHGSESRFDFRKYIQRSLEEDFKTVVEISPVIWF VAVLFLLTNTNGLRSYLWLPFIPLIVILIVGTKLQVIITKLGLRIQEKGDVVRGAPVVQPGDDLFWFGKPRFILFLIHLVLFTNAFQLAFFAWSTYEFNLRNCFHESNADVVIRIAVGVVVQILCSYVTLPLYALVTQMGTKMKPTVFNERVATALKKWHHTAKSQTKHGRHSESNTPFSSRPTTPTHGSSPIHLLHNFNNRNVDSYPTSPSPRYSGHGHHDEHQFWDPESQRQEAETSTHHSLAHESSERKPALASVELPPIRTSKSLKDFSFKR核苷酸序列：ATGGCGAATCAAGTTAAGGAGCGGACTTTAGAAGAGACCTCTACGTGGGCAGTAGCTGTGGTTTGCTTCGTCTTACTATTTATTTCGATTGTTCTCGAACATTCGATTCACAAAATTGGAACCTGGTTTAGAAAGAAGCACAAGAAGGCTCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGCTGTTGGGATTCATATCACTACTCCTCACTATTGGACAAACGCCAATCTCAAATATCTGCATCTCCGAGCAGGTTGCATCGACAATGCACCCTTGCAGCGCCGCGGAAGAAGCTAGAAAATACCACAAGAAAGACCCCGCAAAGAAAGAACATGGAGATGGAGATGGAGAAAAATCGGGTCGAAGACTTCTTCTTGAGTTAGCTGAATCTTATATCCCTAGACGAAGTTTAGCCACCAAAGGTTATGACAAATGTGCAGAGAAGGGGAAAGTGGCTTTTGTATCTGCTTATGGAATCCACCAGTTGCATATATTCATCTTTGTGCTGGCCGTGGTTCATGTTATTTATTGCATTGTTACTTATGCTCTCGGAAAGACTAAGATGAGGAGGTGGAAGTCATGGGAGGAGGAGACGAAGACAATAGAGTATCAGTATTCCAATGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCCTTTGGGAGAAGACATCTCAATTTCTGGAGTAAGTCGAGTGTTACACTATGGGTTGTGTGTTTTTTCAGACAGTTCTTTGGATCTGTCACCAAAGTTGATTACTTAGCACTAAGACATGGTTTCATCATGGCGCATTTGGCTCACGGGAGCGAATCAAGATTTGATTTCCGCAAGTATATTCAGAGATCATTAGAGGAAGACTTCAAAACTGTTGTTGAAATCAGTCCGGTTATTTGGTTTGTCGCGGTGCTATTCCTCTTGACCAATACAAATGGACTACGTTCTTACCTTTGGTTACCATTTATTCCACTAATCGTAATTCTAATAGTTGGAACAAAGCTTCAAGTCATAATTACCAAATTGGGTCTAAGAATCCAAGAGAAAGGTGATGTGGTGAGAGGTGCTCCGGTGGTTCAGCCTGGTGATGACCTCTTCTGGTTTGGCAAGCCACGGTTCATTCTTTTTCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTAGCCTTCTTTGCCTGGAGTACGTATGAGTTCAATCTCAGGAATTGTTTCCATGAAAGCAATGCAGATGTGGTCATTAGGATTGCAGTTGGAGTTGTTGTGCAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCAGATGGGTACTAAGATGAAGCCAACAGTATTTAACGAAAGAGTAGCCACAGCATTAAAGAAGTGGCATCACACGGCAAAGAGCCAGACTAAACACGGAAGACACTCTGAATCAAACACACCTTTCTCTAGCCGTCCAACCACACCAACACATGGCTCATCTCCCATCCATCTCCTTCACAATTTCAATAACCGGAACGTTGACAGTTACCCAACTTCTCCTTCTCCTAGATACTCTGGTCATGGTCATCATGATGAACACCAGTTTTGGGATCCTGAGTCTCAACGCCAAGAAGCTGAAACGTCCACACATCATTCTCTTGCGCATGAAAGCTCAGAAAGGAAGCCGGCTCTTGCATCTGTTGAACTTCCTCCAATAAGGACTAGCAAAAGCTTAAAAGATTTCTCTTTTAAGAGATGAcarubv10020030m.g氨基酸序列：MAEQVKEKTLEETSTWAVAVVCFVLLLISIVIENLIHKIGSWFKNKNKKALYEALEKVKAELMLMGFISLLLTIGQGYISNICIPKNIAASMHPCSAAEEARKYGKKLVPKVQEDGGDDDDNSRRKLLQLVDSIIPRRSLATKGYDKCAEKGKVAFVSAYGMHQLHIFIFVLAVCHVIYCIVTYALGKTKMRRWKRWEQETKTIEYQYSHDPERFRFARDTSFGRRHLNFWSRTTVTLWIVCFFRQFFRSVTKVDYLTLRHGFIMAHLAPGSDARFDFRKYIQRSLEEDFKTVVEINPVIWFVAVLFLLTNTNGLNSYLWLPFIPFVVILIVGTKLQVIITKLGLRIQEKGDVVKGTPLVQPSDHFFWFGRPRFILFLIHLVLFTNAFQLAFFVWSTYEFGLKNCFHESSVDVIIRISIGLIVQILCSYVTLPLYALVTQMGSKMKPTVFNERVAIALKSWHHTAKKQIKHGRTSESTTPFSSRPTTPTHGSSPIHLLRNVPHKRSRSVDDNSFANSMSPRRNSDFDSWDPESQHETAETSTNSNHRSRFREEESEKKLGPSSSVELPPGPGQIRTQHDISNISLRDFSFKR核苷酸序列：ATGGCGGAACAAGTGAAAGAAAAAACATTAGAGGAAACTTCTACATGGGCCGTCGCTGTGGTTTGCTTCGTCTTGCTTCTGATTTCTATTGTTATTGAGAATCTTATTCATAAAATTGGATCCTGGTTTAAAAACAAGAACAAAAAAGCTTTATATGAAGCTCTTGAAAAAGTGAAAGCAGAGCTTATGCTGATGGGATTCATATCACTACTGCTTACTATTGGACAAGGCTACATCTCGAATATTTGCATCCCTAAGAACATCGCAGCGTCGATGCACCCTTGTAGTGCAGCCGAAGAAGCGAGAAAGTACGGTAAGAAATTAGTCCCAAAGGTCCAGGAAGACGGAGGAGACGACGATGACAACTCGCGTCGAAAGCTTCTCCAATTAGTCGATTCTATCATTCCTCGAAGGAGTTTGGCTACCAAAGGTTATGATAAGTGTGCAGAGAAGGGGAAAGTCGCTTTTGTATCGGCTTATGGCATGCATCAGCTGCATATATTCATCTTTGTTCTTGCGGTTTGTCATGTGATTTACTGCATTGTTACTTATGCTTTGGGGAAGACCAAGATGAGAAGGTGGAAGAGGTGGGAACAGGAGACGAAGACAATCGAATATCAGTATTCACACGATCCCGAGAGGTTTAGGTTCGCGAGGGATACATCTTTCGGGCGTAGACATCTGAATTTCTGGAGCAGAACAACTGTTACGCTGTGGATTGTATGTTTCTTCAGACAGTTCTTTAGATCAGTAACCAAAGTTGATTACTTAACACTGAGACATGGTTTCATCATGGCTCATTTGGCTCCTGGGAGCGATGCAAGATTCGATTTCCGAAAGTACATTCAGAGATCATTAGAGGAAGACTTTAAAACCGTTGTCGAGATCAATCCTGTGATCTGGTTCGTCGCTGTCTTGTTTCTCCTGACCAACACAAACGGATTGAATTCTTACCTCTGGTTACCTTTCATTCCTTTTGTCGTGATTCTTATCGTTGGGACAAAACTTCAAGTGATAATAACAAAACTAGGACTACGAATCCAAGAGAAAGGCGACGTAGTAAAAGGCACACCGCTTGTTCAGCCCAGTGATCATTTCTTCTGGTTCGGTCGCCCACGTTTCATTCTCTTCCTCATTCACTTGGTTCTCTTCACGAACGCGTTTCAACTCGCTTTCTTTGTCTGGAGTACGTATGAATTCGGTCTAAAGAACTGTTTCCATGAAAGCAGTGTAGATGTAATCATCAGAATCTCAATCGGACTTATTGTCCAGATTCTTTGCAGCTACGTTACTCTTCCTCTTTACGCTCTTAGTCACTCAGATGGGTTCAAAGATGAAACCAACAGTGTTCAACGAGAGAGTAGCAATAGCATTAAAGAGTTGGCACCACACAGCAAAGAAACAGATCAAACATGGAAGAACCTCAGAATCAACCACACCTTTCTCTAGCCGACCAACAACACCAACACATGGTTCTTCTCCGATTCATCTCCTCCGCAACGTCCCTCACAAACGAAGCAGAAGCGTTGACGATAACAGCTTTGCGAATTCAATGTCTCCGAGAAGAAACTCTGATTTCGATTCGTGGGATCCTGAGTCTCAACACGAAACTGCTGAGACTTCGACGAACTCAAATCATCGTTCAAGGTTTAGAGAAGAAGAATCGGAGAAAAAGCTTGGACCTTCTTCATCTGTTGAACTTCCTCCTGGACCTGGTCAGATACGAACTCAGCATGATATTAGTAATATCAGCTTAAGGGATTTTTCGTTTAAGCGGTGA。