| 主权项 |
1. A method of diagnosis of a brain damage-related disorder selected from stroke and sub-arachnoid hemorrhage in a subject comprising:
(a) detecting a level of expression of glutathione S transferase P and at least one polypeptide selected from the group consisting of peroxiredoxin 1, peroxiredoxin 2, peroxiredoxin 5, and peroxiredoxin 6, or variants or mutants thereof in a sample of plasma, serum or cerebrospinal fluid taken from the subject, wherein the variants or mutants have a homology of at least 90% with the polypeptide; (b) detecting a level of expression of the glutathione S transferase P and the polypeptide in a control sample of plasma, serum or cerebrospinal fluid taken from a control subject known not to have suffered from the brain damage-related disorder; and (c) diagnosing the subject as having suffered from the brain damage-related disorder when the level-of expression of glutathione S transferase P and the at least one polypeptide in the sample is higher than the level of expression in the control sample, and wherein detecting the level of expression in the sample (a) and the control sample (b) comprises: immunodepletion of abundant proteins in the sample or the control sample; and assaying the immunodepleted sample or control sample using an antihuman GST-P antibody and an antibody that recognizes, binds to or has affinity for a polypeptide selected from the group consisting of peroxiredoxin 1, peroxiredoxin 2, peroxiredoxin 5 and peroxiredoxin 6 and mutants or variants thereof in the sample or the control sample, wherein the variants or mutants have a homology of at least 90% with the polypeptide. |
