| 发明名称 | Ketoreductase-mediated stereoselective route to alpha chloroalcohols | ||
| 摘要 | The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of α-chloroalcohols from α-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides. | ||
| 申请公布号 | US9029112(B2) | 申请公布日期 | 2015.05.12 |
| 申请号 | US201414313465 | 申请日期 | 2014.06.24 |
| 申请人 | Codexis, Inc. | 发明人 | Bong Yong Koy;Vogel Michael;Collier Steven J.;Mitchell Vesna;Mavinahalli Jagadeesh |
| 分类号 | C07H21/00;C12N15/00;C12N5/10;C12N1/21;C12P21/00;C12P7/28;C12P13/02;C12P17/02;C12N9/04;C12N9/02 | 主分类号 | C07H21/00 |
| 代理机构 | Codexis, Inc. | 代理人 | Codexis, Inc. |
| 主权项 | 1. A recombinant polynucleotide encoding an engineered ketoreductase polypeptide capable of converting (S)-tert-butyl 4-chloro-3-oxo-l-phenylbutan-2-ylcarbamate to tert-butyl (2S,3R)-4-chloro-3-hydroxy-l-phenylbutan-2-ylcarbamate with a relative activity at least 1.2-fold that of the polypeptide of SEQ ID NO:2, wherein the engineered ketoreductase polypeptide comprises an amino acid sequence at least 90% identical to SEQ ID NO:2 and includes one or more amino acid substitutions relative to SEQ ID NO:2, selected from the group consisting of: P2L; V28A; A34S; A47V; E50K; D81N; S90V; I91L; I91W; I91R; I91K; K94R; D112Y; G117D; S143R; V144T; G145A; R148H; A150G; F152L; N153G; N153V; N153H; T158S; G190A; S198N; I199M; I199L; I199N; M200I; A217T; I225V; P231F; A232V; E233Q; D244G; F260Y; and S261N. | ||
| 地址 | Redwood City CA US | ||