Modified aminotransferase, gene thereof, and method for producing optically active amino compound using same

摘要

Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.

主权项

1. A polypeptide having the following properties (i) to (iii):
(i) having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1, wherein one or more amino acids have been substituted, deleted or inserted in said sequence; (ii) being capable of catalyzing a reaction of 7-methoxy-2-tetralone to produce (S)-7-methoxy-2-aminotetralin in the presence of an amino group donor; and (iii) having greater enzymatic catalytic activity than that of an aminotransferase consisting of the amino acid sequence of SEQ ID NO: 1.