RECOMBINANT PLASMID DNA pCLm4/hygro-14D5 CODING POLYPEPTIDE WITH LIGHT CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND RECOMBINANT PLASMID DNA pCHm2-14D5 CODING POLYPEPTIDE WITH HEAVY CHAIN PROPERTIES OF CHIMERIC ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, CHIMERIC ANTIBODY PROVIDING ACUTE PREVENTION OF TICK-BORNE ENCEPHALITIS IN MICE

摘要

FIELD: medicine, pharmaceutics.SUBSTANCE: group of inventions refers to biotechnology, gene and protein engineering. There are engineered plasmids pCLm4/hygro-14D5 and pCHm2-14D5. The plasmids provide the synthesis in eukaryotic cells of polypeptides with light and heavy chain properties of a chimeric antibody, which are combined into the chimeric antibody ch14D5 class IgG1/kappa. The producing chimeric antibody ch14D5 recovered from the culture fluid of CHO-K1 cells transfected by plasmids pCLm4/hygro-14D5 and pCHm2-14D5 having a molecular weight of about 150 kDa. The antibody consists of two identical light chains, a constant portion of which corresponds to human kappa antibodies, and two identical heavy chains, a constant portion of which corresponds to human IgGI antibodies; having an amino acid sequence SEQ ID NO: 1 and SEQ ID NO: 2.EFFECT: antibody is targeted to tick-borne encephalitis and provides the acute prevention of mice against tick-borne encephalitis.3 cl, 7 dwg, 8 ex