发明名称 |
UROKINASE-TYPE PLASMINOGEN ACTIVATOR TRANSGENIC MOUSE |
摘要 |
The present invention provides a mouse with liver damage, having a high degree of damage against the mouse's original hepatocytes while having a uPA gene in a heterozygous form, and a method for efficiently preparing the mouse. Specifically, the method for preparing a mouse with liver damage having the uPA gene in a heterozygous form comprises the following steps of:;(i) transforming mouse ES cells with a DNA fragment containing a liver-specific promoter/enhancer and cDNA that encodes a urokinase-type plasminogen activator operably linked under the control thereof;
(ii) injecting the transformed mouse ES cells obtained in step (i) into a host embryo;
(iii) transplanting the host embryo obtained in step (ii) via the injection of the ES cells into the uterus of a surrogate mother mouse, so as to obtain a chimeric mouse; and
(iv) crossing the chimeric mice obtained in step (iii), so as to obtain a transgenic mouse in which the DNA fragment is introduced in a heterozygous form. |
申请公布号 |
US2015128298(A1) |
申请公布日期 |
2015.05.07 |
申请号 |
US201314397091 |
申请日期 |
2013.04.25 |
申请人 |
TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE ;CHUGAI SEIYAKU KABUSHIKI KAISHA ;PHOENIXBIO CO., LTD. |
发明人 |
Kohara Michinori;Jishage Koichi;Kawase Yosuke;Mukaidani Chise;Oshita Hiroki;Hamamura Satoko |
分类号 |
A01K67/027;C12N15/877;C12N15/85;G01N33/50 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Tokyo JP |