Methods and Compositions for Identifying Sulfur and Iron Modifying Bacteria

摘要

The invention relates to methods and compositions for identifying specific bacterial species, which are sulfur and iron oxidizers and/or reducers, from wall board (e.g., dry wall) and/or a patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the bacterial species from the wall board and/or the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the bacterial species, and specifically identifying the bacterial species. Kits and nucleic acids for use in the methods are also provided. Methods for eliminating the sulfur and iron oxidizing and/or reducing bacteria from wall board using a zeolite are also provided.

主权项

1. A method of identifying a specific iron and sulfur reducing and/or oxidizing bacterial species in wall board, the method comprising the steps of:
extracting and recovering DNA of the bacterial species from the wall board, amplifying the DNA; hybridizing a probe to the DNA to specifically identify the bacterial species; and specifically identifying the bacterial species, wherein the probe, a forward primer, and a reverse primer are used during the amplification step, and wherein the probe comprises a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 7, SEQ ID NO: 10, SEQ ID NO: 13, and SEQ ID NO: 16.