METHOD FOR THE CHEMICAL SURFACE ACTIVATION OF A SOLID SILICON-BASED SUPPORT BY MEANS OF DIRECT COVALENT ANCHORING OF AT LEAST ONE BIOMOLECULE OF NUCLEIC ACIDS

摘要

The invention relates to a method for chemical surface activation, by a thiol-ene (TEC) or thiol-yne (TYC) reaction, of a solid silicon-based support by means of direct covalent anchoring of at least one biomolecule which is a sequence of DNA or RNA oligonucleotides, where a) the surface of the support is functionalised with alkene or alkyne groups and the biomolecule has a terminal thiol group, and/or b) the surface of the support is functionalised with thiol groups and the biomolecule has a terminal alkene or alkyne group. Said method comprises the steps of: depositing the sequence of oligonucleotides on the surface of the support by means of contactless or contact printing techniques; and anchoring the sequence of oligonucleotides to the surface of the support by radical addition by means of a TEC or TYC reaction caused by photoirradiation with near-visible UV light over an interval of between 5 minutes and 3 hours, without requiring the use of crosslinkers and/or catalysts.