| 摘要 |
The present invention provides a method for determining an amount of nucleic acid molecule copies of a preselected nucleic acid molecule in a sample in a fragmentation based method, comprising the steps of providing the fragment amount for said preselected nucleic acid molecule, a distribution of fragment coverage over a genetic coordinate for said preselected nucleic acid molecule, obtaining a plurality of virtual fragments, assembling the obtained virtual fragments, obtaining the amount of assembled virtual fragments per assembled sequence, whereby the amount of the copies of a preselected nucleic acid molecule in a sample can be calculated from the fragment quantitiy and the amount of assembled virtual fragments per assembled sequence. |
