Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity

摘要

The present invention relates to thermostable or thermoactive DNA polymerases with attenuated 3'-5' exonuclease ("proofreading") activity, methods for their synthesis, methods for their use, and kits comprising them. The enzymes are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).