Anti-cancer agent comprising anti-HB-EGF antibody as active ingredient

摘要

A monoclonal antibody having a neutralizing activity on HB-EGF is disclosed. The monoclonal antibody of the present invention is preferably an antibody that does not bind to the HB-EGF protein on the cell surface of HB-EGF-expressing cells. Also provided are an anti-cancer agent and a cell proliferation inhibitor, which comprise the monoclonal antibody of the present invention as an active ingredient, and a method of treating cancer, the method comprising administering the monoclonal antibody of the present invention. Cancers that can be treated by the anti-cancer agent of the present invention include pancreatic cancer, liver cancer, esophageal cancer, melanoma, colorectal cancer, gastric cancer, ovarian cancer, bladder cancer, and brain tumors.

主权项

1. An isolated monoclonal antibody that has a neutralizing activity on HB-EGF, which does not bind to the HB-EGF protein on the cell surface of cells that express HB-EGF having SEQ ID NO:60 wherein the antibody binds to the secreted forth of HB-EGF, which comprises the heparin-binding domain and the EGF-like domain of the HB-EGF protein, and wherein the antibody binds to an epitope that is the same as the epitope of the antibody comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO:2 as CDR1, the amino acid sequence of SEQ ID NO:4 as CDR2, and the amino acid sequence of SEQ ID NO:6 as CDR3 and comprising a light chain variable region having the amino acid sequence of SEQ ID NO:12 as CDR1, the amino acid sequence of SEQ ID NO:14 as CDR2, and the amino acid sequence of SEQ ID NO:16 as CDR3.