Liposome and method for injecting substance to cell using this liposome

摘要

It is intended to efficiently inject into a target cell, a substance charged within a liposome. The present inventors have found that connexin synthesized within a liposome is introduced as connexon having a gap junction function into the liposome membrane. Specifically, the liposome according to the present invention is a liposome in which connexon composed of connexin synthesized by an in-vitro protein synthesis system is incorporated in a state of having a gap junction function.

主权项

1. A liposome comprising:
a connexon composed of oligomeric connexin co-translationally inserted into a connexon-free phospholipid bilayer of said liposome, wherein said connexin is synthesized by in-vitro protein synthesis and assembled into oligomers in the phospholipid bilayer of said liposome, wherein said connexon forms a gap junction with an opposing cell, and wherein the liposome maintains an intended substance without transmitting it to the outside before formation of said gap junction.