发明名称 |
RNA-guided human genome engineering |
摘要 |
A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. |
申请公布号 |
US9023649(B2) |
申请公布日期 |
2015.05.05 |
申请号 |
US201414319100 |
申请日期 |
2014.06.30 |
申请人 |
President and Fellows of Harvard College |
发明人 |
Mali Prashant G.;Church George M.;Yang Luhan |
分类号 |
C12N15/85;C12N9/14;C12N9/22;C07H21/02;C07H21/04;C12N15/87;C12N15/01;C12N15/81;C12N15/82 |
主分类号 |
C12N15/85 |
代理机构 |
Banner & Witcoff, Ltd. |
代理人 |
Banner & Witcoff, Ltd. |
主权项 |
1. A method of altering a eukaryotic cell comprising
providing to the eukaryotic cell guide RNA sequences complementary to target nucleic acid sequences, providing to the cell a Cas9 protein having inactive nuclease domains that interacts with the guide RNA sequence and binds to the target nucleic acid sequences in a site specific manner, wherein the Cas 9 has a FokI nuclease domain attached thereto, wherein a target nucleic acid sequence is cleaved upon FokI nuclease domain dimerization of adjacent guide RNA sequence and Cas9 protein co-localization complexes. |
地址 |
Cambridge MA US |