FGL-2 prothrombinase as a diagnostic tool for malignancy

摘要

The present invention reveals a strong correlation between FGL-2 prothrombinase activity levels and the presence of a malignant proliferative disorder in a subject. Thus, the present invention provides FGL-2 prothrombinase activity as a diagnostic tool for malignancy.

主权项

1. A method for the diagnosis of a malignant proliferative disorder in a subject, said method comprising the steps of:
(a) collecting a blood sample from said subject and a blood sample from a normal control; (b) isolating peripheral blood mononuclear cells (PBMC) from said samples; (c) measuring prothrombinase activity in the PBMC from said samples, using a thrombin generation assay, comprising generating thrombin from added prothrombin and determining the thrombin by using a chromogenic substrate; and; (d) comparing the prothrombinase activity of the PBMC from said subject with the prothrombinase activity from said control;whereby a sample with prothrombinase activity higher than control is indicative of the presence of a malignant disorder in said subject.