Hair follicle graft from tissue engineered skin

摘要

The present invention provides a hair graft comprising (a) tissue engineered skin comprising a tissue engineered epidermal layer, a tissue engineered dermal layer, and hair follicle progenitor cells and (b) a scaffold. The invention also provides methods of making and using the hair grafts of the present invention.

主权项

1. A method of making a hair graft, comprising: a) incubating a tissue engineered dermal layer on a bioabsorbable scaffold until the tissue engineered dermal layer attaches to the bioabsorable scaffold; b) placing hair follicle progenitor cells on the tissue engineered dermal layer; c) placing a tissue engineered epidermal layer comprising a sheet of keratinocytes on the hair follicle progenitor cells to form a construct; d) allowing the construct to mature in vitro; and e) cutting the construct into one or more implantable grafts.