METHOD AND SYSTEM FOR THE ANALYSIS OF HIGH DENSITY CELLS SAMPLES

摘要

Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyses on the formed cell arrays.

主权项

1. A method for forming a cell array comprising a plurality of discrete cell samples on a single substrate, the method comprising the steps of:
(a) mixing a cell suspension in a source to obtain a substantially uniform distribution of cells within the cell suspension; (b) aspirating the cell suspension from the source; (c) movably transporting the cell suspension relative to the substrate; (d) metering a predetermined amount of the cell suspension using a positive displacement pump; and (e) dispensing the metered amount of the cell suspension in the form of droplets onto the substrate so as to form a plurality of discrete cell samples, each discrete cell sample arranged substantially in a monolayer on the single substrate.