Microfluidic Liposome Synthesis, Purification and Active Drug Loading

摘要

Microfluidic methods and systems are provided for continuous flow synthesis and active loading of liposomes, which include a liposome formation region configured to form a population of liposomes and a microdialysis region downstream from the liposome formation region and configured to form a transmembrane gradient for active drug loading of the liposomes. Microfluidic methods and systems for high throughput production of liposomes are also provided featuring high aspect ratio microchannels.

主权项

1. A microfluidic system for continuous flow synthesis and active loading of liposomes, comprising:
a substrate comprising a sample flow channel having a liposome formation region, a transmembrane gradient formation region, and an agent loading region, wherein:
said liposome formation region comprises an inlet through which a lipid solution flows and inlets through which a buffer solution flows, said lipid solution and said buffer solution interacting within said sample flow channel and forming a population of liposomes in a sample buffer solution;said transmembrane gradient formation region configured to establish a liposome transmembrane gradient; andsaid agent loading region comprises an inlet through which a first agent flows and in fluid connection with said sample flow channel, said first agent mixed with said liposomes received from said transmembrane gradient formation region and actively loaded within intravesicular spaces of said liposomes.