发明名称 |
METHOD FOR DELIVERY OF SMALL MOLECULES AND PROTEINS ACROSS THE CELL WALL OF ALGAE USING MOLECULAR TRANSPORTERS |
摘要 |
The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell. |
申请公布号 |
US2015118704(A1) |
申请公布日期 |
2015.04.30 |
申请号 |
US201314396274 |
申请日期 |
2013.03.12 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY ;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
Geihe Erika;Trantow Brian;Wender Paul;Hyman Joel M.;Parvin Bahram |
分类号 |
C12N1/12;C12Q1/24 |
主分类号 |
C12N1/12 |
代理机构 |
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代理人 |
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主权项 |
1. A method of delivering a cargo compound to an algal cell, the method comprising contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell; and allowing the guanidinium-rich molecular transporter to traverse the algal cell wall, thereby delivering the cargo compound to the algal cell. |
地址 |
Palo Alto CA US |