KISSPEPTIN-54 DETECTION BY TANDEM MASS SPECTROMETRY

摘要

Methods are described for measuring the amount of a kisspeptin-54-derived peptides in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying a kisspeptin-54 derived peptides in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric techniques.

主权项

1. A method for determining the presence of or risk of developing preeclampsia by mass spectrometry, said method comprising:
(a) subjecting a body fluid or tissue sample obtained from a patient to ionization under conditions suitable to produce one or more multiply charged kisspeptin-54-derived peptide ions detectable by mass spectrometry; (b) determining by mass spectrometry the amount of one or more multiply charged ions selected from the group of ions with a charge consisting of 4+, 5+, 6+, and 7+ from each of said one or more kisspeptin-54-derived peptides; and (c) using the amount of the determined ions to determine the amounts of the corresponding one or more kisspeptin-54-derived peptides in the sample.wherein elevated levels of one or more kisspeptin-54-derived peptides in the sample relative to normal levels indicate the presence of or risk of developing preeclampsia.