Compositions Comprising Near-Infrared Fluorescent Particles And Uses Thereof For Imaging Activated Immune Cells In the CNS

摘要

Pharmaceutical composition including nanoparticles configured for enhanced phagocytosis by phagocytic cells and labeled with a near-infrared (NIR) fluorescent probe bound to the outer surface thereof, and uses thereof in the detection of activated immune cells in the central nervous system (CNS) of a subject.

主权项

1. A method for detecting activated phagocytic cells in the central nervous system (CNS) of a subject, the method comprising the steps of:
(i) parenterally administering to a subject a pharmaceutical composition comprising nanoparticles configured for enhanced phagocytosis by phagocytic cells, the nanoparticles characterized by at least one structural or physicochemical feature that enhances their uptake by phagocytic cells compared to equivalent nanoparticles without the at least one structural or physicochemical feature, the nanoparticles further comprising a near-infrared (NIR) fluorescent probe; (ii) irradiating at least a portion of the CNS of the subject with NIR radiation having a wavelength that is absorbed by the NIR fluorescent probe; and (iii) detecting NIR fluorescence emission from the probe, wherein a locality of said fluorescence emission from the probe is indicative of a locality of activated phagocytic cells, thereby detecting activated phagocytic cells in the CNS of the subject.