DNA METHYLATION ANALYSIS FOR THE DIAGNOSIS, PROGNOSIS AND TREATMENT OF ADRENAL NEOPLASMS

摘要

Disclosed herein are methods for detecting, diagnosing and/or prognosing a malignant adrenocortical tumor. Also disclosed are methods of treating a malignant adrenocortical tumor, such as ACC. In some examples, the method of diagnosing and/or prognosing includes obtaining a sample comprising genomic DNA from a subject at risk of acquiring or suspected to have an adrenocortical tumor; isolating genomic DNA from the sample; and measuring the level of one or more methylated genomic CpG dinucleotide sequences in one or more of the adrenocortical genomic targets in the sample, wherein an increase in the level of methylation of the one or more genomic CpG dinucleotide sequences in the sample compared to a control indicates a malignant adrenocortical tumor.

主权项

1. A method for diagnosing a malignant adrenocortical tumor, comprising:
obtaining a sample comprising genomic DNA from a subject at risk of acquiring or suspected to have an adrenocortical tumor; isolating genomic DNA from the sample; and measuring the level of one or more methylated genomic CpG dinucleotide sequences within one or more of malignant adrenocortical tumor-related molecules in the sample, wherein the one or more malignant adrenocortical tumor-related molecules comprise at least one of SLC16A9, IL13RA2, HTR2B, CCNB2, RARRES2, KCTD12, KIRREL, SYNGR1 or NTNG2, and wherein an increase in the level of methylation of the one or more genomic CpG dinucleotide sequences within one or more of malignant adrenocortical tumor-related molecules compared to a control diagnoses the subject with a malignant adrenocortical tumor.