发明名称 |
SWITCHABLE CAS9 NUCLEASES AND USES THEREOF |
摘要 |
Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity and/or improving the specificity of RNA-programmable endonucleases, such as Cas9. For example, provided are guide RNAs (gRNAs) that are engineered to exist in an "on" or "off state, which control the binding and hence cleavage activity of RNA-programmable endonucleases. Some aspects of this disclosure provide mRNA- sensing gRNAs that modulate the activity of RNA-programmable endonucleases based on the presence or absence of a target mRNA. Some aspects of this disclosure provide gRNAs that modulate the activity of an RNA-programmable endonuclease based on the presence or absence of an extended DNA (xDNA). |
申请公布号 |
WO2015035139(A3) |
申请公布日期 |
2015.04.30 |
申请号 |
WO2014US54252 |
申请日期 |
2014.09.05 |
申请人 |
PRISIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
LIU, DAVID, R.;HU, JOHNNY, HAO |
分类号 |
C12N15/113 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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