| 发明名称 |
CHYMOSINE ENZYME VARIANTS |
| 摘要 |
A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295
said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese. |
| 申请公布号 |
US2015118356(A1) |
申请公布日期 |
2015.04.30 |
| 申请号 |
US201314397866 |
申请日期 |
2013.05.03 |
| 申请人 |
DSM IP ASSETS B.V. |
发明人 |
Dekker Petrus Jacobus Theodorus;De Jong René Marcel;Muijlwijk Cornelis Marinus |
| 分类号 |
A23C19/032;C12N9/64 |
主分类号 |
A23C19/032 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids of positions
2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. |
| 地址 |
Heerlen NL |
