Magnetic-assisted rapid aptamer selection method for generating high affinity DNA aptamer

摘要

A magnetic-assisted rapid aptamer selection (MARAS) protocol for screening DNA aptamer is proposed. The MARAS protocol is able to efficiently generate aptamers with high affinity and specificity. A rotating magnetic field or alternating magnetic field was used in combination with target-bound magnetic micro-particles or nanoparticles to select DNA aptamers having desirable affinity and specificity to the target.