发明名称 |
Method for enhancing enzyme assays |
摘要 |
Additives capable of controlling the reaction rate between substrate and enzyme to form a dye in enzyme assays with absorbance, chemiluminescence, and fluorescence are effective tools for optimizing enzyme assays. A dye can be formed rapidly from the reaction between enzyme and substrate in the presence of a catalyst. Using relatively high concentration of dye formed from the rapid reaction, trace levels of analytical materials can be quantified using absorbance, chemiluminecence and fluorescence detection. A dye can be formed from a relatively slow reaction between enzyme and substrate in the presence of a surfactant such as Triton X-100 and β-cyclodextrin. Using relatively low concentration of dye from the slow reaction, a high concentration of analytical material can be quantified without any dilution using absorbance, chemiluminescence, and fluorescence. |
申请公布号 |
US9017950(B2) |
申请公布日期 |
2015.04.28 |
申请号 |
US201213422650 |
申请日期 |
2012.03.16 |
申请人 |
Luminescent MD, LLC |
发明人 |
Lee Ji Hoon |
分类号 |
G01N33/53;G01N33/533;C12Q1/28;G01N33/535;G01N33/542 |
主分类号 |
G01N33/53 |
代理机构 |
Birch, Stewart, Kolasch & Birch, LLP |
代理人 |
Birch, Stewart, Kolasch & Birch, LLP |
主权项 |
1. A method of enhancing sensitivity of a chemiluminescent immunoassay, comprising reacting 10-Acetyl-3,7-dihydroxyphenoxazine with hydrogen peroxide in the presence of horseradish peroxidase and p-Iodophenol, wherein the chemiluminescent immunoassay further comprises 1,1′-Oxalyldi-4-methyl-imidazole (OD4MI). |
地址 |
MD US |