Microscope and a fluorescent observation method using the same

摘要

A microscope capable of controlling the position and fluorescent recording of an object under observation such as cells is provided with the fluorescent observation method using the microscope. The microscope 1 comprises: a stage 3 on which the object under observation 2 is placed; an illumination light source 4 for the object under observation 2; an excitation light source 5 for exciting fluorescent light F to the object under observation 2; an image information detecting part 16 for detecting the image information formed with the light T generated at the object under observation 2; a fluorescent image information detecting part 17 for detecting the fluorescent image information formed with fluorescent light F; and a control part 20, which determines the fluorescent observation area of the object under observation 2 based on the dynamic model of the object under observation 2 and its image information entered from the image information detecting part 16, and then obtains the image information of the object under observation 2 entered from the image information detecting part 16 and the fluorescent image information entered from the fluorescent image information detecting part 17 at specified interval within the fluorescent observation area.

主权项

1. A microscope for analyzing a biological tissue, comprising:
a stage for placing an object under observation that is the biological tissue; a first light source with an optical system for radiating illumination light to the object under observation; a second light source with an optical system for radiating excitation light for exciting fluorescent light to the object under observation; an image detecting part for detecting an image of the object under observation formed by the illumination light; a fluorescent image detecting part for detecting a fluorescent image of the biological tissue formed by the fluorescent light, said stage, the fluorescent image detection part, and the second light source being configured to capture a fluorescent image of a horizontal cross section of the biological tissue and a vicinity thereof at multiple levels along a height of the biological tissue; and a control part that determines a fluorescent observation portion of the biological tissue from which said fluorescent image of said horizontal cross section is taken at the multiple vertical levels, wherein the control part determines the fluorescent observation portion by:
determining a center of the fluorescent observation portion along a vertical direction using at least either one of the image of the biological tissue detected by the image detecting part or a dynamic model of the biological tissue; anddetermining a vertical length of the fluorescent observation portion by a classic control law that is PID control law or by modern control law that is any one of optimum control law and sub-optimum control law applied to the image detected by the image detecting part, wherein, after determining the fluorescent observation portion, the control part causes the image detecting part and the fluorescent image detecting part to detect an image of the biological tissue and a fluorescent image of a horizontal cross section of the biological tissue at multiple levels in the vertical direction within the fluorescent observation portion, said multiple levels being distributed in the vertical length of the fluorescent observation portion at a predetermined interval.