Devices and methods for infrared (IR) based quantitation of biomolecules

摘要

The present invention provides methods for quantitating one or more biomolecules in a sample using IR based techniques, sample holder devices for use in such methods as well as methods for manufacturing such sample holder devices.

主权项

1. A method for quantitation of one or more biomolecules in a sample, the method comprising the steps of:
(a) providing a sample holder comprising a porous membrane which comprises a hydrophilic region surrounded by a hydrophobic region for sample containment; (b) contacting the hydrophilic region of the membrane with a sample volume, wherein the hydrophilic region has a diameter; (c) drying the sample volume on the membrane; (d) exposing the sample volume on the membrane to an infrared beam having a diameter equal to or larger than the diameter of the hydrophilic region, and comprising a wavelength in the spectral range of 4000-400 cm−1 or any portion of the spectral range of 4000-400 cm−1, thereby to obtain an infrared absorption spectrum;
wherein one or more absorption peak areas in the infrared absorption spectrum correlates with the quantity of the one or more biomolecules in the sample.