Gamma satellite insulator sequences and their use in preventing gene silencing

摘要

Regulatory elements, specifically insulators and transgene constructs containing insulator nucleic acid sequences, are disclosed herein. Methods of using insulators and transgene constructs including insulators to inhibit, delay, or prevent gene silencing are also disclosed herein.

主权项

1. A method of inhibiting silencing of a coding nucleic acid sequence to be expressed in a recipient cell, comprising: introducing into a chromosomal site in the recipient cell a transgene construct comprising a coding nucleic acid sequence to be expressed in the recipient cell, wherein the coding nucleic acid sequence is flanked on each of its 5′ and 3′ ends by at least six repeats of a human gamma-satellite DNA monomer that is heterologous to the coding nucleic acid sequence, and wherein the at least six repeats of a human gamma-satellite DNA monomer inhibits silencing of expression of the coding nucleic acid sequence, thereby inhibiting silencing of the coding nucleic acid sequence to be expressed in the recipient cell.