Reagents for probing ROCKs activation

摘要

A set of agents is provided for probing/detecting the activation of Rho-associated protein kinase I and II individually encoding their amino acid sequence (SEQ ID NO:1 for ROCKI and SEQ ID NO:2 for ROCKII) in biological samples. The agents include one antibody to specifically recognize ROCKI Ser1333 phosphorylation and one antibody to specifically recognize ROCKII Ser1366 phosphorylation. The agents can be used for pathological analysis in cardiovascular diseases, cancer, neurological diseases or other diseases/disorders involving ROCKs and also for basic medical research. Moreover, the agents can be used for screening inhibitors against ROCKI and II.

主权项

1. A reagent for probing the activation of a Rho-associated protein kinase II (ROCKII) in a biological sample from a mammal, the ROCKII comprising a peptide-II sequence, wherein the reagent comprises an antibody-II that specifically binds to a phosphorylated serine amino acid at position 1366 in SEQ ID NO:2 but does not bind when serine amino acid at position 1366 in SEQ ID NO:2 is not phosphorylated.