Production of a viable, storable worm egg suspension

摘要

The present application describes a novel, advantageous purification method which guarantees the embryonation capability and the viability of a worm egg suspension. The T. suis worm eggs are first treated in a non-embryonated condition in a sulfuric acid solution at about =ph 2. Bacteria and viruses are thereby killed successfully. The germination number of yeasts and fungi is reduced. In a second step, the pH-value is increased and an orally tolerated preservative is added. Yeasts and fungi are thereby killed successfully. The suspension medium used in the second purification step can then be used as a culture medium for additional production (embryonation) and storage.

主权项

1. A process for the preparation of a non-pathogenic pharmaceutical preparation of a storable suspension of viable parasitic Trichuris suis eggs for oral administration comprising:
obtaining viable eggs from Trichuris suis; subjecting said eggs to sulphuric acid treatment at a pH of ≦2 for a period of 2 to 8 hours; adjusting the pH to ≧4 by adding a suitable base; and adding a pharmacologically acceptable preservative, wherein after the ingestion of the suspension of eggs by a human, a sufficient number of helminths develop which result in a stimulation of regulatory human T-cells.