Hairless NOD scid mouse

摘要

Hairless, immunodeficient mice on a non-obese diabetic (NOD) background and methods for their production are disclosed herein. The mice are hairless and have multiple immunodeficiencies, including B-cell and T-cell deficiencies, as well as impaired macrophage and complement function. The mice also have a further deficit in natural killer and dendritic cells of the immune system. The mice are useful for biomedical research, for example, in studies involving xenograft transplantation, spontaneous tumors, cancer cell tumorigenesis, tumor angiogenesis, tumor metastatic potential, tumor suppression therapy, carcinogenesis regulation, and tumor imaging.

主权项

1. A method of producing a transgenic, hairless, immunodeficient mouse whose genome comprises a homozygous Prkdcscid mutation and a homozygous Hrhr mutation on a non-obese diabetic NOD.CB17 background, the method comprising:
(a) crossing a homozygous NOD.CB17-Prkdcscid mouse with a homozygous MF-1-hr mouse to produce a progeny that is heterozygous for both the Hr allele and the Prkdc allele; (b) crossing the heterozygous progeny produced from step (a) with NOD.CB17-Prkdcscid mice; (c) selecting an offspring of step (b) that has a NOD.CB17 background, is homozygous for the Prkdc allele, and is heterozygous for the Hr allele; (d) crossing the offspring selected in step (c) with mice homozygous or heterozygous for the Hr allele to obtain a homozygous NOD.CB17-Prkdcscid Hrhr double mutant transgenic mouse, wherein the NOD.CB17-Prkdcscid Hrhr double mutant transgenic mouse exhibits B-cell, T-cell, NK-cell, macrophage, and complement deficiencies, and a reduction in the number of dendritic cells compared to an immunodeficient mouse of a NOD.CB17-Prkdcscid background that is not hairless.