DETECTION OF SUB-CELLULAR COMPARTMENT LOCALIZATION OF A MOLECULE USING A REDUCED AFFINITY ENZYME COMPLEMENTATION REPORTER SYSTEM

摘要

Methods and compositions for detecting the sub-cellular localization of a molecule are provided. Aspects of the invention include detecting translocation of a cell-surface receptor to a sub-cellular compartment, e.g., the endosome, using a reduced affinity enzyme complementation reporter system. Also provided are systems and kits for use in practicing embodiments of the methods.