发明名称 |
Modular DNA-binding domains and methods of use |
摘要 |
The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell. |
申请公布号 |
US9017967(B2) |
申请公布日期 |
2015.04.28 |
申请号 |
US201414153241 |
申请日期 |
2014.01.13 |
申请人 |
|
发明人 |
Bonas Ulla;Boch Jens;Schornack Sebastian;Lahaye Thomas |
分类号 |
C12N5/071;C12N1/20;C12N5/04;C07K14/195 |
主分类号 |
C12N5/071 |
代理机构 |
Williams Mullen PC |
代理人 |
Williams Mullen PC ;Saravitz David M. |
主权项 |
1. A non-human animal cell or in vitro human cell comprising a fusion protein, the fusion protein comprising an artificial transcription activator-like (TAL) effector repeat domain of contiguous repeat units 33 to 35 amino acids in length and an endonuclease domain, wherein the repeat domain is engineered for recognition of a predetermined nucleotide sequence in the non-human animal cell or in vitro human cell, and wherein the fusion protein is capable of recognizing the predetermined nucleotide sequence in the non-human animal cell or in vitro human cell. |
地址 |
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