| 摘要 |
PROBLEM TO BE SOLVED: To provide an endoglycosidase which has an activity having a wide substrate specificity, cleaving and releasing the N-glycan from glycoprotein, and transferring the sugar chain, and to provide an enzyme which does not have an activity cleaving and releasing the N-glycan from glycoprotein, and only has an activity transferring the sugar chain.SOLUTION: The invention provides: an endoglycosidase derived from Coprinus cinereus consisting of a specific amino acid sequence which has an activity cleaving the N-glycan from glycoprotein to release the sugar chain, and transferring the sugar chain; and an enzyme derived from Coprinus cinereus consisting of a specific amino acid sequence which does not have an activity cleaving the N-glycan from glycoprotein to release the sugar chain, and only has an activity transferring the sugar chain. |
