| 摘要 |
<p>An isolated mutant cocaine esterase (CocE) polypeptide comprising an amino acid sequence at least 85% identical to SEQ ID NO: 1, wherein SEQ ID NO: 1 has at least one substitution, addition or deletion selected from the group consisting of L163V, V225I, I218L, A310D, A149S, S159A, S265A, S56G, W220A, S140A, F189L, A193D, T254R, N42V, V262L, L508G, Y152H, V160A, T172R, Y532F, T74S, W285T, L146P, D533S, A194R, G173Q, C477T, K531A, R41I, L119A, K46A, 10 F84Y, T172R-G173Q, L169K, F189A, N197K, R182K, F189K, V190K, Q191K, A194, I175-G-G-A186, and T176-G-G-D18; and the mutant CocE polypeptide has esterase activity with increased thermostability at 37°C as compared to wild-type CocE, wherein SEQ ID NO: 1 is disclosed within the specification.</p> |
