Compositions and methods for identifying and differentiating viral components of multivalent shipping fever vaccines

摘要

Disclosed is a composition adapted for detecting a BVDV-1b viral-specific nucleic acid, if present, within a plurality of attenuated, live virus particles, comprising: (a) a first viral-specific oligonucleotide amplification primer of less than about 50 nucleotides in length comprising a nucleotide sequence of SEQ ID NO:1; (b) a second viral-specific oligonucleotide amplification primer of less than about 50 nucleotides in length comprising a nucleotide sequence of SEQ ID NO:2; and (c) at least a first labelled, viral-specific detection probe of less than about 50 nucleotides in length comprising a labelled nucleotide sequence of SEQ ID NO:3; wherein the sequences are as defined in the specification; and wherein the composition is adapted for detecting a viral-specific nucleic acid using quantitative, real time polymerase chain reaction (qPCR).