| 摘要 |
<p>Disclosed is a method of inactivating a lipid-coat containing virus, the method comprising the steps of: (a) obtaining a provided fluid comprising a Factor VIII having an activity; (b) mixing tri(n-butyl) phosphate, polyoxyethylene octyl phenyl and polysorbate 80 sorbitan with the fluid thereby creating a mixture, wherein the final concentration of tri(n-butyl) phosphate is from about 0.1 % (v/v) to about 5.0% (v/v), the final concentration of polyoxyethylene octyl phenyl is from about 0.5% (v/v) to about 10.0% (v/v), and the final concentration of polysorbate 80 sorbitan is from about 0.1 % (v/v) to about 5.0% (v/v); and (c) incubating the mixture obtained from step (b) for no more than about 120 minutes, wherein both steps (b) and (c) are performed at a temperature of between about 2 degrees C and about 8 degrees C, wherein the mixture after incubation is essentially free of a viable lipid-coat containing virus, and wherein the Factor VIII after incubation has an activity of at least 25%, at least 50% or at least 75% of the activity provided in step (a).</p> |
