| 摘要 |
The invention relates to a method for calibrating an infrared spectroscopy apparatus in order to produce spectra with a view to determining the concentration of a component C1 in one fluid F1 of an animal, which includes the following steps: a) obtaining samples of said fluid F1 and of a fluid F2 other than the fluid F1 from each animal belonging to a group of representative animals, the fluid F2 containing detectable amounts of at least one component C2 which is directly or indirectly related to a metabolic pathway of the component C1; b) measuring the concentration of the component C1 in the samples of fluid F1 by means of a reference method; c) producing the complete IR absorption spectrum of the samples of fluid F2; d) identifying in the absorption spectra of the samples of fluid F2 the spectral ranges that correlate with the concentration of the component C1 in the samples of fluid F1; and e) calculating, on the basis of at least one of said correlating spectral ranges, a predictive mathematical model of the concentration of the component C1 in the fluid F1. The method is used for predicting the level of components in a first fluid from the dose of components in another fluid. The method is also used to identify compounds involved in a metabolic pathway. |
