METHOD OF INDUCING KIDNEY FROM PLURIPOTENT STEM CELLS

摘要

The purpose of the present invention is to provide a process or method that can be utilized when deriving a three-dimensional structure of a kidney from pluripotent stem cells such as ES cells or iPS cells. This differentiation inducing method is characterized by culturing pluripotent cells with the following three steps, in order: (a) a step in which an embryoid body induced from the pluripotent stem cells is cultured in a culture medium that contains Bmp4 and a high-concentration (concentration A) Wnt agonist; (b) a step in which the embryoid body is cultured in a culture medium which contains activin, Bmp4, retinoic acid and a middle-concentration (concentration B) Wnt agonist; and (c) a step in which the embryoid body is cultured in a culture medium that contains Fgf9 and a low-concentration (concentration C) Wnt agonist (herein, the Wnt agonist concentrations satisfy concentration A > concentration B > concentration C).